Journal article
Self-organized nanostructure modified microelectrode for sensitive electrochemical glutamate detection in stem cells-derived brain organoids
B Nasr, R Chatterton, JHM Yong, P Jamshidi, GM D'Abaco, AR Bjorksten, O Kavehei, G Chana, M Dottori, E Skafidas
Biosensors | MDPI | Published : 2018
DOI: 10.3390/bios8010014
Abstract
Neurons release neurotransmitters such as glutamate to communicate with each other and to coordinate brain functioning. As increased glutamate release is indicative of neuronal maturation and activity, a system that can measure glutamate levels over time within the same tissue and/or culture system is highly advantageous for neurodevelopmental investigation. To address such challenges, we develop for the first time a convenient method to realize functionalized borosilicate glass capillaries with nanostructured texture as an electrochemical biosensor to detect glutamate release from cerebral organoids generated from human embryonic stem cells (hESC) that mimic various brain regions. The biose..
View full abstractGrants
Awarded by Centre of Excellence for Integrative Brain Function, Australian Research Council
Funding Acknowledgements
This work was supported by the Australian Research Council Centre of Excellence for Integrative Brain Function (ARC Centre Grant CE140100007). M. Dottori is supported by the Australian Research Council Future Fellowship. The fabrication of glutamate biosensors were partly performed at the Melbourne Centre for Nanofabrication (MCN) in the Victorian Node of the Australian National Fabrication Facility (ANFF). The authors also acknowledge the facilities and technical assistance of the Micro Nano Research Facility (MNRF) at RMIT. The authors acknowledge the opportunity to use the facilities of School of Physics at The University of Melbourne.